In Sakura Knight 2, players join Estelle in her determined pursuit to emulate her mother, the legendary knight Cressida. Following the events of the first game, Estelle has regained her mother's sword and assembles a vibrant team of companions to aid her in her quest for fame and recognition in the bustling city of Grimoire, Lemuria's largest metropolis.

Accompanied by the lovable yet scatterbrained elf Felicia, the playful catgirl Tart, and the caring wolfgirl Rune, Estelle embarks on various quests offered by the guild. Together, they battle against monstrous foes including wicked plant and slime creatures, all while honing Estelle's swordplay skills. However, the journey is not without its challenges, as Tart and Rune's constant bickering threatens to derail their progress and frustrate their mission.

Additionally, Estelle must uncover the truth behind a mysterious woman who appears to have a hidden agenda against her. Amidst the chaos, Estelle is also faced with choices about whom to ultimately dedicate her heart to. Will she rise to the occasion and become the knight she's always dreamed of being?